the stimulatory effect of mip-1α on the gene transduction efficacy of cord blood cd34+ cells by a pseudotype retroviral vector

introduction: hematopoietic stem cells are always in a quiescence state. since they need retroviral transduction to infect dividing cells, they are resistant to retrovirus transduction. they need to be pre-stimulated by a cytokine cocktail. for proliferation without maturation, we suggest mip-1α as a novel factor. material and methods: retroviral vector produced by pg13/ln c8 cells titter on hela cells. then, the cd34 + cells of cord blood can be pre-stimulated in a serum- free media supplemented with scf, flt3,tpo,il6 in the presence and absence of 50 ng/ml mip-1 α . transduction efficiency was assessed by a semi-quantitative pcr for the neomycin gene. results: a pcr analysis of the neomycin gene in cd34 + cells revealed an improved transduction of cord blood cells in the presence of mip-1 α 65%, in comparison to its absence: 40.7%. conclusion: the addition of mip-1α to the cytokine cocktail improves the transduction efficiency of cord blood hematopoietic progenitor cells. further studies are required to clarify its effect on the functional properties of cd34 + cells.